产品展示首页 > 试剂盒 > 试用装申请 > 试用装申请
RunBlue 预制胶 10*10cm
货    号:
NTB41212
规    格:
25ml
品    牌:
expedeon(英国)
英文名称: RunBlue Bis-Tris Protein Gels 4-12%
特异性: RunBlue 预制凝胶与目前常用的SDS-PAGE凝胶相比有许多改进,RunBlue凝胶比传统预制凝胶更坚硬,有高于10倍的强度,不用再担心凝胶被撕裂! 优点:1. 质谱兼容的 ;2 .2年保质期,不用担心过期了;3.比传统凝胶强度大10倍--没有撕裂,毁坏的凝胶;4.没有梳子要移除--不再有损坏或弯曲的齿;5.容易打开,手指就行--不再需要弯曲的刮刀
实验原理:

RunBlue Bis-Tris Protein Gels have been developed by Expedeon to provide an alternative to NuPAGE® Bis-Tris gels. RunBlue Bis-Tris Protein gels run with the same voltages and times, in the same tanks, using the same MES/MOPS buffers as NuPAGE® Bis-Tris gels and achieve highly comparable results with exceptional reproducibility. RunBlue Bis-Tris Protein Gels also benefit from the usability enhancements present in all RunBlue gels such as comb/strip free design and tear proof composition. 

  • Comparable results to NuPAGE® Bis Tris Gels 

  • Same protocols, buffers, and gel tank

  • More practical comb free, strip free, tear proof gels

RunBlue Features & Benefits

  •  Standard Bis-Tris Buffers – Compatible with MES and MOPS buffers
  •  Standard format – Compatible with 10x10cm cassette tanks such as NuPAGE® XCell SureLock™ Mini-Cell
  •  Standard Bis-Tris Protocol – Fast run times at 200V
  •  Neutral running pH – Sharp bands and high protein integrity
  •  Unique homogeneous polymerisation – Increased consistency and no residual free acrylamide
  •  Extra Wells – Run up to 12 or 17 samples simultaneously
  •  Deep and wide wells – Load up to 35 μl (12 well) or 20 μl (17 well)
  •  Protruding teeth – No well contamination in case of overloading
  •  Resilient gel matrix – Tear-proof gel and risk-free handling
  •  Comb-free – Reduced risk of broken or damaged teeth
  •  Strip-free – No potentially ruined Western Blots
  •  Neutral buffer and high chemical stability – Shelf life of 2 years at 4°C and 3 months at RT

Additional Information

Application No
Storage +4C
Shelf Life 2 Years

RunBlue Performance

RunBlue Bis-Tris Protein Gels are based on Bis-Tris neutral gel buffer system, providing a migration profile highly comparable to Nupage® Bis-Tris gels. RunBlue Bis-Tris Protein Gels are also compatible with the NuPAGE® XCell SureLock™ Mini-Cell running tanks and MES/MOPS buffers. RunBlue Bis-Tris Protein Gels have an approximate range of 3.5kDa to 160kDa with MES Buffer and 30kDa to 180kDa when using MOPS. RunBlue Protein Gel’s proprietary polymerisation process results in more uniform gels between batches, with decreased variability and improved repeatability of results. These gels take advantage of Expedeons new methods and innovations to the gel making process to increase resolution and reproducibility of separated protein bands.

 

Usability Enhancements

In addition to their high resolution separation of protein all RunBlue Protein Gels have a number of enhancements to improve and streamline usability. RunBlue Protein Gels are up to 10 times stronger than conventional hand-cast gels and can stand up to repeated handling. This practically eliminates the risk of torn and unusable gels encountered during normal gel manipulation. More lanes than standard gels allows more proteins to be run and wide, deep wells allow higher volumes to be loaded.

 

Cassettes have no comb or tape to remove and no excess gel to trim, all of which improves the speed and efficiency of the loading and running process. Wells are outlined and numbered for quicker sample loading and the cassette is labelled with gel type, percentage and a unique lot number for traceability. Well teeth protrude above the cassette to prevent sample contamination between wells in case of overloading.


Flexible Usage

RunBlue Bis-Tris Protein Gels are highly optimised for a MES/MOPS buffer system and must be run with these buffers. Usage of MES or MOPS will vary depending on the molecular weight range of proteins to be resolved. RunBlue Bis-Tris Protein Gels are must be run with MES or MOPS buffers such as RunBlue MES Running Buffer and RunBlue MOPS Running Buffer which are available as a premixed 20X solution. And the samples must be prepared with a Tris-HCl LDS sample buffer such as RunBlue LDS Bis-Tris Sample buffer which is available as a premade 4X solution. RunBlue Bis-Tris Protein Gels are also compatible with Nupage® MES and MOPS buffers and NuPAGE® LDS Sample Buffer. RunBlue Protein Gels have an extended shelf life of 2 years at 4°C which is ideal for large stocks or infrequent use. These gels are compatible with Invitrogen® XCell SureLock™ Mini-cell equipment to minimise hassle if Expedeon gels are used. They also have a 3 month shelf life at room temperature which is convenient when fridge space is limited.

 

Improved Safety

Polyacrylamide gels are based on the polymerisation of liquid acrylamide and bis-acrylamide. The polymerisation is usually induced by the addition of a source of free radicals and a stabilizer (usually APS & TEMED) which induces cross link formation between acrylamide and bis-acrylamide monomers. This localised chemical induction results in heterogeneous polymerisation across the gel due to acrylamide concentration gradients being generated, and can lead to the presence of residual free acrylamide at the end of the polymerisation.


Expedeon has developed a unique physical induction technology which ensure homogenous polymerisation across the whole gel and no residual free acrylamide after the end of the polymerisation. Numerous studies have shown the acrylamide monomer is a neurotoxin and possible carcinogen*. Acrylamide can be inhaled as a dry powder, evaporate from liquid solution and sublimate from solid gels if not fully polymerised. RunBlue gels are polymerised in a unique homogenous manner that results in a consistent gel with minimal or no residue after casting.


组成:
现货: 上海现货
运输条件: 常温
保存条件: 2-8°C
有效期: 12个月
Copyright © 2013 上海武昊生物科技有限公司 All Rights Reserved 版权所有 鲎试剂 ELISA
电话:+86-21-68509247 邮编:200136 传真:021-58680811
企业邮箱:销 售 部:wuhao@wuhaosh.cn orders@wuhaosh.cn info@wuhaosh.cn sales@wuhaosh.cn 沪ICP备:11021032号-2       

沪公网安备 31011502005524号